August 27, 2024 Stephen Gunstream Chief Executive Officer Alpha Teknova, Inc. 2451 Bert Dr. Hollister, CA 95023 Re: Alpha Teknova, Inc. Registration Statement on Form S-1 Filed August 23, 2024 File No. 333-281765 Dear Stephen Gunstream: This is to advise you that we have not reviewed and will not review your registration statement. Please refer to Rules 460 and 461 regarding requests for acceleration. We remind you that the company and its management are responsible for the accuracy and adequacy of their disclosures, notwithstanding any review, comments, action or absence of action by the staff. Please contact Jimmy McNamara at 202-551-7349 with any questions. Sincerely, Division of Corporation Finance Office of Life Sciences cc: Elizabeth Razzano