United States securities and exchange commission logo November 2, 2023 Stephen Gunstream President and Chief Executive Officer Alpha Teknova, Inc. 2451 Bert Dr. Hollister, CA 95023 Re: Alpha Teknova, Inc. Registration Statement on Form S-1 Filed October 25, 2023 File No. 333-275168 Dear Stephen Gunstream: This is to advise you that we have not reviewed and will not review your registration statement. Please refer to Rules 460 and 461 regarding requests for acceleration. We remind you that the company and its management are responsible for the accuracy and adequacy of their disclosures, notwithstanding any review, comments, action or absence of action by the staff. Please contact Cindy Polynice at 202-551-8707 with any questions. Sincerely, Division of Corporation Finance Office of Life Sciences cc: Elizabeth Razzano, Esq.